国产91亚洲福利精品一区二区,国产综合成人久久大片91,国产成人精品久久综合,久久久91精品国产一区二区三区,91福利国产在线在线播放,91精品国产高清久久久久久91,91精品国产福利在线观看麻豆,国产免费一区二区三区在线观看

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > PYS-2
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
PYS-2
PYS-2
規(guī)格:
貨期:
編號(hào):B212468
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 PYS-2
商品貨號(hào) B212468
Organism Mus musculus, mouse
Tissue yolk sac; parietal endoderm
Cell Type epithelial
Product Format frozen
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease carcinoma
Age embryo
Storage Conditions liquid nitrogen vapor phase
Genes Expressed
osteonectin (BM-40)
Cellular Products
osteonectin (BM-40)
Comments
SPARC is an extracellular Ca+2 binding glycoprotein, which is differentially associated with morphogeneisis, remodeling, cellular migration and proliferation [PubMed; 2745554]. Exogenous SPARC, added to cells in culture is associated with profound changes in cell shape, caused rapid, partial detachment of a confluent monolayer, and inhibited spreading of newly plated cells [PubMed; 2745554].
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Name of Depositor EH Sage
Deposited As mouse
References

Sage H, et al. SPARC, a secreted protein associated with cellular proliferation, inhibits cell spreading in vitro and exhibits Ca+2-dependent binding to the extracellular matrix. J. Cell Biol. 109: 341-356, 1989. PubMed: 2745554

Basu A, et al. The intracisternal A-particle proximal enhancer-binding protein activates transcription and is identical to the RNA- and DNA-binding protein p54nrb/NonO. Mol. Cell. Biol. 17: 677-686, 1997. PubMed: 9001221

Satyamoorthy K, et al. Identification of an intronic enhancer that nullifies upstream repression of SPARC gene expression. Nucleic Acids Res. 25: 3169-3174, 1997. PubMed: 9224620

Lehman JM, et al. Neoplastic differentiation: characteristics of cell lines derived from a murine teratocarcinoma. J. Cell. Physiol. : 13-27, 1974. PubMed: 4858519

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
兴隆县| 桑植县| 兴义市| 左云县| 大余县| 保德县| 吴忠市| 凤山县| 广元市| 安徽省| 邵阳市| 大同县| 天峨县| 东乡县| 灌云县| 铜陵市| 故城县| 兰考县| 德阳市| 乐至县| 峡江县| 瑞金市| 云南省| 永仁县| 嘉峪关市| 龙游县| 镇巴县| 屏东市| 濮阳县| 喀喇| 惠安县| 五常市| 墨玉县| 名山县| 营口市| 赤水市| 荃湾区| 图木舒克市| 祁门县| 特克斯县| 克什克腾旗|