国产91亚洲福利精品一区二区,国产综合成人久久大片91,国产成人精品久久综合,久久久91精品国产一区二区三区,91福利国产在线在线播放,91精品国产高清久久久久久91,91精品国产福利在线观看麻豆,国产免费一区二区三区在线观看

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > pFL46L
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
pFL46L
pFL46L
規(guī)格:
貨期:
編號:B210479
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pFL46L
商品貨號 B210479
Designations pFL46L
Depositors F Lacroute
Biosafety Level 1
Vector Information
Size (kb): 7.8220000267028810
Vector: pFL46L (plasmid)
Construction: pUC19
Marker(s):LEU2,ampR
Construct size (kb): 7.822000026702881
Features: marker(s): ampR, LEU2
replicon: pMB1, 2 micron
Applications
YE-type (episomal) shuttle vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--7.8; BglII--4.9, 2.9; ClaI--3.7, 2.3, 1.9.
Contrary to published data, this vector does not permit visual detection of recombinants using the assay for alpha complementation.
Shows increased stability because of the presence of STB. The loss per generation (percentage having lost all the plasmids after 20 h of non-selective growth) is 6.3.
YE-type high copy number shuttle vector.
The order of the major features in this plasmid is: NotI/MCS/HindIII - LEU2 - STB -2 micron ori - pMB1 ori - ampR.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Yanisch-Perron C, et al. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119, 1985. PubMed: 2985470

Struhl K, et al. High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules. Proc. Natl. Acad. Sci. USA 76: 1035-1039, 1979. PubMed: 375221

Bonneaud N, et al. A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors. Yeast 7: 609-615, 1991. PubMed: 1767589

Chevallier MR, et al. Transcriptional and translational expression of a chimeric bacterial-yeast plasmid in yeasts. Gene 11: 11-19, 1980. PubMed: 7002730

Francois Lacroute, personal communication

Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
盐源县| 如东县| 大丰市| 广西| 名山县| 涿鹿县| 朝阳县| 田东县| 资溪县| 繁峙县| 南宫市| 长汀县| 溧阳市| 舞钢市| 顺义区| 济南市| 册亨县| 磴口县| 天等县| 博客| 上思县| 伽师县| 乌鲁木齐县| 大洼县| 搜索| 基隆市| 金塔县| 大同市| 四川省| 会同县| 额尔古纳市| 兴仁县| 平塘县| 邳州市| 河间市| 普宁市| 潮安县| 邢台市| 淳安县| 合川市| 威海市|