It is important to check whether the strain is [rho+] or [rho-] prior to use for complementation analyses.
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen
Type Strain
no
Genotype
MATa pet17 leu2 leu1 trp1 thr1 arg4 lys7 ura3 his5 ade5 gal1 CUP(r)
RefHawthorne DC, Mortimer RK. Genetic mapping of nonsense suppressors in yeast. Genetics 60: 735-742, 1968. PubMed: 5732415
Preceptrol®
no
Mating Type
a
Ploidy
haploid
Comments
Respiration: uncharacterized nuclear petite genes (pet) (CE1)
Genetic petites become [rho-] at a high frequency. It is important to check whether the strain is [rho+] or [rho-] prior to use for complementation analyses.
Medium
ATCC® Medium 1069: YPAD medium
Growth Conditions
Temperature: 25.0°C
Atmosphere: Typical aerobic
Name of Depositor
YGSC
Special Collection
Yeast Genetic Stock Center
Chain of Custody
ATCC <<--YGSC<<--R.K. Mortimer
References
Hawthorne DC, Mortimer RK. Genetic mapping of nonsense suppressors in yeast. Genetics 60: 735-742, 1968. PubMed: 5732415
Hawthorne DC, Mortimer RK. Genetic mapping of nonsense suppressors in yeast. Genetics 60: 735-742, 1968. PubMed: 5732415