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pUCD2 plasmid in E. coli
pUCD2 plasmid in E. coli
規(guī)格:
貨期:
編號:B209795
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pUCD2 plasmid in E. coli
商品貨號 B209795
Designations pUCD2 plasmid in E. coli
Depositors CI Kado
Biosafety Level 1
Vector Information
Size (kb): 13.0
Vector: pUCD2 (plasmid)
Promoters: Promoter none
Construction: pBR322, pSal151
Marker(s):ampR,kanR,spcR,tetR
Construct size (kb): 13.0
Features: marker(s): ampR, kanR, spcR, tetR
promoter: none
replicon: pMB1, pSa151
terminator: none
enhancer: none
Applications
shuttle vector
Comments
A broad host range amplifiable cloning vector.
This is a broad host range amplifiable cloning vector for use in complementation studies of cloned inserts in Agrobacterium tumefaciens.
It was constructed by fusing pBR322 with pSAl51 plasmid and was shown to have lost 2.5 kb on each side of the BglII site giving it a positive vir region complementation in Agrobacterium tumafaciens.
Restriction digests of the clone give the following sizes (kb): PstI--13; EcoRV--13; KpnI--13; SalI--13.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Close TJ, et al. Design and development of amplifiable broad-host-range cloning vectors: analysis of the vir region of Agrobacterium tumefaciens plasmid pTiC58. Plasmid 12: 111-118, 1984. PubMed: 6095350

Shipped freeze-dried
Shipping Information

Shipped: ?Freeze dried E. coli containing the cosmid

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