国产91亚洲福利精品一区二区,国产综合成人久久大片91,国产成人精品久久综合,久久久91精品国产一区二区三区,91福利国产在线在线播放,91精品国产高清久久久久久91,91精品国产福利在线观看麻豆,国产免费一区二区三区在线观看

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > Sarcocystis neurona
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
<em>Sarcocystis neurona</em>
<em>Sarcocystis neurona</em>
規(guī)格:
貨期:
編號:B209737
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Sarcocystis neurona
商品貨號 B209737
Strain Designations SN3.E1
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation Spinal cord homogenate from horse (2-yr-old thoroughbred filly), Panama, circa 1991.
Product Format frozen
Storage Conditions Frozen Cultures:
-70°C for 1 week; liquid N2 vapor for long term storage

Freeze-dried Cultures:
2-8°C

Live Cultures:
See Protocols section for handling information
Genome Sequenced Strain

Yes

Comments Causative agent of equine protozoal myeloencephalitis (EPM)
Growth Conditions Temperature: 37 °C
Atmosphere: 5% CO2
Cell line: Bovine turbinate cells (ATCC® CRL-1390™)
Cryopreservation Harvest and Preservation
  1. Harvest the Sarcocystis culture by gently agitating the contents of each flask.  Transfer all but approximately 1 mL of the culture medium to 15 mL plastic centrifuge tubes. Detach the remaining infected and uninfected tissue culture cells by scraping the inner surface of the flask with a cell scraper. Pass the resulting cell suspension through a syringe equipped with a 27-gauge ½-inch needle and pool this suspension with the culture fluid.
  2. Spin the cell suspensions at approximately 50 x g for 3 min, to remove the cellular debris.
  3. Transfer the supernatants to new 15 mL plastic centrifuge tubes.  Centrifuge at 1300 x g for 10 min.
  4. Pool the parasite pellets and adjust the concentration to ~2.0 x 107 merozoites/mL with fresh Advanced MEM.
    *If the concentration is too low, centrifuge at 1300 x g for 10 min and resuspend in the volume of Advanced MEM required to yield the desired parasite concentration.
  5. Mix equal volumes of parasite suspension and fresh medium containing 20% DMSO and 50% HIFBS to yield a final concentration of ~1 x 107 merozoites/mL in 10% DMSO, 25% HIFBS.  The time from the mixing of the parasite preparation and the cryoprotective solution before the freezing process begins should be no less than 15 min and no more than 30 min.
  6. Dispense in 0.5 mL aliquots to 1.0-2.0 mL sterile plastic screw-capped cryovials.
  7. Place the vials in a controlled rate freezing unit.  From room temperature, cool at -1°C/min to -40°C.  If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion.  At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.  Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.  The cooling rate in this apparatus is approximately -1°C/min.  
  8. Store in either the vapor or liquid phase of a nitrogen refrigerator.
  9. To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes.  Do not agitate the ampule.  Do not leave ampule in water bath after thawing.
  10. Immediately after thawing, aseptically transfer contents to a T-25 tissue culture flask containing a fresh monolayer of ATCC® CRL-1390™ cells and 10 mL of Advanced MEM with 4% (v/v) HIFBS.
  11. Outgas the flask for 10 seconds with a 95% air, 5% CO2 gas mixture.
  12. Incubate in a 35-37°C CO2 incubator with the caps screwed on tightly.
Name of Depositor DK Howe
Cross References

Nucleotide (GenBank) : JAQE00000000 Sarcocystis neurona strain SN3 clone E1, whole genome shotgun sequencing project

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
安塞县| 镇平县| 金坛市| 雅安市| 金寨县| 嘉祥县| 中江县| 克拉玛依市| 竹北市| 聂拉木县| 文登市| 乾安县| 黄浦区| 梓潼县| 上思县| 兴文县| 浦江县| 关岭| 柘荣县| 清水县| 芜湖市| 龙里县| 若尔盖县| 文山县| 北流市| 鲜城| 将乐县| 西乡县| 吉安县| 嘉峪关市| 炎陵县| 德惠市| 盐源县| 安阳市| 毕节市| 长顺县| 荣成市| 白城市| 安平县| 乌鲁木齐市| 开封县|