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HO-poly-HO
HO-poly-HO
規(guī)格:
貨期:
編號:B209470
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 HO-poly-HO
商品貨號 B209470
Designations HO-poly-HO
Depositors DJ Stillman
Biosafety Level 1
Vector Information
Size (kb): 4.5999999046325680
Vector: HO-poly-HO (plasmid)
Promoters: Promoter HO
Construction: pUC21
Marker(s):ampR
Construct size (kb): 4.599999904632568
Features: promoter: HO
Applications
cloning vector
Comments
Restriction digests of the clone give the following sizes (kb): XbaI--4.6; NotI--3.0, 1.6.
A 912 bp PCR product with HindIII and BsiWI ends and containing HO promoter sequences (-1720 to -1814 upstream from the ATG) was inserted into the HindIII-BsiWI digested pUC21-NotI.
A 507 bp PCR product with EcoRI and SfiI ends and containing 3' end sequences of HO gene (1199 to 1699 downstream from the ATG) was inserted into the EcoRI-SfiI digested pUC21-NotI.
This plasmid allows targeted integration at HO locus.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
Protocol: (LB + amp)
References

Voth WP. Yeast vectors for integration at the HO locus.. Nucleic Acids Res. 29(12): E59-9, 2001. PubMed: 11410682

David J Stillman, personal communication

Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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