国产91亚洲福利精品一区二区,国产综合成人久久大片91,国产成人精品久久综合,久久久91精品国产一区二区三区,91福利国产在线在线播放,91精品国产高清久久久久久91,91精品国产福利在线观看麻豆,国产免费一区二区三区在线观看

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > pRS422
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
pRS422
pRS422
規(guī)格:
貨期:
編號:B209247
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pRS422
商品貨號 B209247
Designations pRS422
GenBank Number

U93719

Depositors JD Boeke
Applications
vector permitting visual detection of recombinants phosphoribosylaminoimidazole succinocarboxamide synthetase SAICAR synthetase, phosphoribosylaminoimidazole carboxylase
Vector
Intact vector size: 6.868
Type of vector: phagemid
Cloning sites: SacI SacII EagI NotI SpeI BamHI SmaI PstI EcoRI ClaI SalI XhoI ApaI KpnI
Polylinker sites: SacI BstXI SacII EagI NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI ApaI KpnI
Construction: pRS402, pRS421
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): ADE2, →, 575-2290
replicon: f1, ←, 2495-2951
promoter for in vitro transcription: T7, →, 3121-3140
MCS: KpnI...SacI, →, 3148-3250
promoter for in vitro transcription: T3, ←, 3267-3286
coding sequence: lacZ', ←, 2952-3311
promoter: lac, ←, 3356-3384
replicon: pMB1, 3710-3710
marker(s): ampR, ←, 4468-5328
replicon: 2 micron, 5460-6801
Vector: pRS422 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pRS402, pRS421
Marker(s): ampR,ADE2
Construct size (kb): 6.868
Features: marker(s): ADE2
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: 2 micron
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
coding sequence: lacZ'
Insert
Target Gene: , phosphoribosylaminoimidazole succinocarboxamide synthetase
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Related Products
Component of: ATCC 87480
Component of: ATCC 87530
Component of: ATCC 87535
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.8; BglII--4.6, 2.2; EcoRI--6.8.
ade2 phenotype produces red colonies when grown on adenine containing media.
This vector is useful for gene knockout experiments in hosts with a non-revertable ade2 auxotrophic marker gene mutation.
References

Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
定边县| 西乌| 高平市| 拜泉县| 荣成市| 石阡县| 上饶市| 南投市| 鄂托克前旗| 玉田县| 合作市| 苍南县| 成都市| 景德镇市| 乌审旗| 昌平区| 泰顺县| 宣恩县| 诸城市| 荥经县| 永兴县| 武定县| 嘉义市| 自治县| 镇原县| 荔浦县| 拉萨市| 朝阳市| 玉树县| 衢州市| 陵川县| 绥中县| 夏津县| 炉霍县| 巩留县| 云霄县| 扶风县| 黄骅市| 拉萨市| 留坝县| 河东区|