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A5.12.14 [5.12.14]
A5.12.14 [5.12.14]
規(guī)格:
貨期:
編號(hào):B209240
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 A5.12.14 [5.12.14]
商品貨號(hào) B209240
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue spleen
Cell Type hybridoma:lymphoblast B lymphocyte; somatic cell hybri
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain: BALB/c (B cell); BALB/c (myeloma)

Storage Conditions liquid nitrogen vapor phase
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Images
Derivation
Animals were immunized with recombinant human interleukin 8 (IL-8). Spleen cells were fused with P3X63Ag8U.1 (ATCC CRL-1597) mouse myeloma cells. The antibody blocks IL-8 binding to human neutrophil receptors. An expression plasmid pantilL-8.2 (ATCC 9056) was created to code for a chimeric murine-human fab fragment of the monoclonal antibody.
Genes Expressed
immunoglobulin; monoclonal antibody; against human interleukin 8 (IL-8)
Cellular Products
immunoglobulin; monoclonal antibody; against human interleukin 8 (IL-8)
Comments
Animals were immunized with recombinant human interleukin 8 (IL-8). Spleen cells were fused with P3X63Ag8U.1 (ATCC CRL-1597) mouse myeloma cells. The antibody blocks IL-8 binding to human neutrophil receptors. An expression plasmid pantilL-8.2 (ATCC 9056) was created to code for a chimeric murine-human fab fragment of the monoclonal antibody.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 10(5) viable cells/ml. Do not allow the cell concentration to exceed 5 X 10(5) cells/ml.
Interval: Maintain cultures at a cell concentration between 3 X 10(4) and 4 X 10(5) cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium described above supplemented with 5% (v/v) DMSO.
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype mouse IgG2a kappa
Name of Depositor Genentech, Inc.
Deposited As mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Fong S, et al. Anti-IL-8 antibody fragments. US Patent 5,677,426 dated Oct 14 1997

Hsei V, et al. Methods of treating inflammatory diseases with anti-IL-8 antibody fragment-polymer conjugates. US Patent 6,468,532 dated Oct 22 2002

Hsei V, et al. Methods of treating inflammatory disease with anti-IL-8 antibody fragment-polymer conjugates. US Patent 6,458,355 dated Oct 1 2002

Gonzalez TN, et al. Humanized anti-IL-8 monoclonal antibodies. US Patent 6,133,426 dated Oct 17 2000

Gonzalez TN, et al. Humanized anti-IL-8 monoclonal antibodies. US Patent 6,117,980 dated Sep 12 2000

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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