Has been used to detect t(10;14)(q24;q11) translocations associated with certain acute T-cell leukemias based on an alteration in the size of the BamHI fragment detected.
Vector
Construct size (kb): 7.699999809265137
Insert
DNA: genomic
Insert lengths(kb): 5.0
Tissue: placenta
Gene product: T cell receptor, delta (V,D,J,C) [TCRD]
Insert Size (kb)
5.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--7.6; EcoRI--5.0, 2.8.
The insert contains the following restriction sites (approximate kb from the 5' end): SacI--1.1, 1.8, 3.0; HindIII--3.5, 4.0.
The MOLT-4 cell line (ATCC CRL-1582) is deleted for this fragment.
Detects the following human genomic germline restriction fragments (kb): BamHI--16.5; HindIII--5.8, 3.2.
Has been used to detect t(10;14)(q24;q11) translocations associated with certain acute T-cell leukemias based on an alteration in the size of the BamHI fragment detected.
Corresponds to the EcoRI fragment between Jdelta1 and Jdelta2.
References
Kagan J, et al. Clustering of breakpoints on chromosome 10 in acute T-cell leukemias with the t(10;14) chromosome translocation. Proc. Natl. Acad. Sci. USA 86: 4161-4165, 1989. PubMed: 2542965
Croce CM. Molecular probing for human T-cell leukemia and lymphoma. US Patent 5,198,338 dated Mar 30 1993
Croce CM. TCL-5 gene rearrangement involved in T-cell leukemia and melanoma. US Patent 5,242,795 dated Sep 7 1993
Disclosure
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