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pE1.8
pE1.8
規(guī)格:
貨期:
編號:B208980
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pE1.8
商品貨號 B208980
Designations pE1.8
Species Homo sapiens, human
Vector
Construct size (kb): 4.800000190734863
Insert
DNA: genomic
Insert lengths(kb): 1.799999952316284
Gene product: ribonucleotide reductase M1 polypeptide(ribonucleoside diphosphate reductase, M1 subunit) [RRM1]
Alleles: A2, A2, A1
Insert Size (kb) 1.800
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
The insert contains the following restriction sites (in nt from the EcoRI site, closer to the XhoI site of the vector): BglII--50; NdeI--201, 907. AatII, ApaI, BamHI, EcoRV, EcoRI, HindIII, Not I, SacI, XbaI and XhoI do not cut the insert.
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.8; BamHI--4.8; SacI--4.8; XhoI--4.8; PstI--uncut.
The 1.8 kb EcoRI fragment (pE1.8, ATCC 65264/65265) is immediately adjacent to and downstream from a 2.8 kb EcoRI fragment (pE2.8, ATCC 65260/65261).
Detects the same 15.5 kb SacI band as pE2.8.
References

Byrne J, Smith P. Human polymorphic probe pE1.8 detects SacI polymorphism in the ribonucleotide reductase M1 subunit gene. Hum. Genet. 87: 376, 1991. PubMed: 1677928

Jennifer A Byrne, personal communication

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