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pLIN101
pLIN101
規(guī)格:
貨期:
編號(hào):B208708
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pLIN101
商品貨號(hào) B208708
Designations pLIN101
GenBank Number

U15050

Species Streptococcus agalactiae Lehmann and Neumann
Depositors DG Pritchard, B Lin
Applications
produces protein hyaluronate lysase
Vector
Construct size (kb): 7.931000232696533
Insert
DNA: genomic
Insert lengths(kb): 3.420000076293945
Gene product: hyaluronate lysase [hylB]
Target Gene: hyaluronate lysase
Insert Size (kb) 3.420
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): SalI--8.0; XbaI--8.0; EcoRI--8.0; BamHI--8.0; SalI/XbaI--4.6, 3.4.
The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--0.35, 0.88; KpnI--1.2; EcoRI--2.19; ClaI--3.22.
The insert excised with SalI/XbaI will include some vector sequence: 12 bp at the 5' end (SalI) and 36 bp at the 3' end (XbaI).
References

Lin B, et al. Cloning and expression of the gene for group B streptococcal hyaluronate lyase. J. Biol. Chem. 269: 30113-30116, 1994. PubMed: 7982914

Pritchard DG, et al. Characterization of the group B streptococcal hyaluronate lyase. Arch. Biochem. Biophys. 315: 431-437, 1994. PubMed: 7986088

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沈經(jīng)理 19548299266 2662369050
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