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pMev
pMev
規(guī)格:
貨期:
編號(hào):B208686
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pMev
商品貨號(hào) B208686
Designations pMev
GenBank Number

M97382

Species Cricetulus griseus, hamster, Chinese
Depositors JL Goldstein
Vector
Construct size (kb): 9.300000190734863
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: Rc/CMV7S
Intact vector size: 6.000
Type of vector: plasmid
Vector end: SalI
Vector end: NotI
Cloning sites:
Polylinker sites:
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, neoR
promoter: CMV
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: hamster, Chinese
Gene symbol: MEVT*
Gene name: mevalonate transporter
Contains complete coding sequence?: U
Tissue: MET-18b-2 cell line
Type of DNA: cDNA
Insert 5' end: SalI
Insert 3' end: NotI
Insert size (kb): 3.345
Cross references: DNA Seq. Acc.: M97382
Insert lengths(kb): 3.34500002861023
Tissue: MET-18b-2 cell line
Gene product: mevalonate transporter(monocarboxylate transporter 1) [MEVT*]
Insert Size (kb) 3.345
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): NotI/SalI--6.0, 3.3; SalI--9.3; NotI--9.3; EcoRI--6.1, 2.2; XhoI--8.0, 1.0, 0.3.
The insert contains the following restriction sites (approximate kb from the 5 ' end): SmaI--0.05, 2.9; EcoRI--2.1; XhoI--2.3.
Transcription of the insert is regulated by the CMV promoter, and transcription of neoR is regulated by the SV40 early promoter.
The ends of the insert were derived from linker adapters.
References

Otto JC, Casey PJ. The hepatitis delta virus large antigen is farnesylated both in vitro and in animal cells. J. Biol. Chem. 271: 4569-4572, 1996. PubMed: 8617711

Kim CM, et al. cDNA cloning of MEV, a mutant protein that facilitates cellular uptake of mevalonate, and identification of the point mutation responsible for its gain of function. J. Biol. Chem. 267: 23113-23121, 1992. PubMed: 1429658

Joseph L Goldstein, personal communication

梅經(jīng)理 17280875617 1438578920
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周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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