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K20PR [TRGV9]
K20PR [TRGV9]
規(guī)格:
貨期:
編號(hào):B208661
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) K20PR [TRGV9]
商品貨號(hào) B208661
Designations K20PR [TRGV9]
GenBank Number

X03436

Species Homo sapiens, human
Vector
Construct size (kb): 3.599999904632568
Insert
DNA: genomic
Insert lengths(kb): 1.029999971389771
Gene product: T cell receptor, gamma, variable region V9 [TCRGV9]
Alleles: A2, A1
Insert Size (kb) 1.030
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.6; HindIII--3.6; PstI--3.6; EcoRI/HindIII--2.5, 1.0; PstI/HindIII--3.6; PstI/EcoRI--2.7, 1.0.
The V9 sequence (without J) is contained in a 410 bp PstI/AccI fragment.
A 1.03 kb PstI/EcoRI fragment isolated from lambdaK20 and containing TCRGV9 gene rearranged to J1, cloned into a pUC vector (the number of the vector is not known).
References

Lefranc MP, et al. Rearrangement of two distinct T-cell gamma-chain variable-region genes in human DNA. Nature 319: 420-422, 1986. PubMed: 3003579

Huck S, Lefranc MP. Rearrangements to the JP1, JP and JP2 segments in the human T-cell rearranging gamma gene (TRGgamma)locus. FEBS Lett. 224: 291-296, 1987. PubMed: 2961609

Forster A, et al. New subgroups in the human T cell rearranging Vgamma gene locus. EMBO J. 6: 1945-1950, 1987. PubMed: 2820713

Marie-Paule Lefranc, personal communication

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