国产91亚洲福利精品一区二区,国产综合成人久久大片91,国产成人精品久久综合,久久久91精品国产一区二区三区,91福利国产在线在线播放,91精品国产高清久久久久久91,91精品国产福利在线观看麻豆,国产免费一区二区三区在线观看

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > pRS317
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
pRS317
pRS317
規(guī)格:
貨期:
編號(hào):B208639
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pRS317
商品貨號(hào) B208639
Designations pRS317
Depositors P Hieter
Biosafety Level 1
Vector Information
Size (kb): 8.30
Vector: pRS317 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pRSS56 [pBluescript KS+, pBS(+)]
Marker(s):ampR,LYS2
Construct size (kb): 8.30
Features: insert detection: lacZ'
marker(s): LYS2
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: ARSH4
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
centromere: CEN6
Applications
YC-type (centromeric) shuttle vector
plasmid shuffling
vector containing primer sites useful for sequencing
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): XbaI--8.4; PstI--8.4; EcoRI--8.4; HindIII--8.4. There are restriction sites for KpnI (2 sites), SpeI (2 sites), and EcoRV (1 site) in the Lys2 gene that are also present in the polylinker.
One of a series of pBluescript-based centromere vectors (ATCC 77142-77145, 77157-77158) differing in the yeast selectable marker gene.
YC-type centromere vector permitting visual detection of recombinants and production of ssDNA in E. coli. Contains promoters for in vitro RNA synthesis, priming sites useful for sequencing, and encodes the lacZ alpha (lacZ') peptide.
Useful in plasmid shuffle experiments.
pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+), contains the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites between bla and f1 origin of pBS(+).
The order of the major features in this plasmid is: LYS2 - f1 ori (NaeI) - T7 promoter - lacZ'/MCS - T3 promoter - pMB1 ori bla - CEN6 - ARSH4.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Sikorski RS, Boeke JD. In vitro mutagenesis and plasmid shuffling: from cloned gene to mutant gene. Methods Enzymol. 194: 302-318, 1991. PubMed: 2005795

Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436

Related Products
component of:ATCC 87562
Shipped frozen
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
静乐县| 陕西省| 泾川县| 台湾省| 冀州市| 那曲县| 三明市| 德化县| 台山市| 汾阳市| 南宫市| 吉木萨尔县| 松溪县| 同仁县| 晋州市| 富锦市| 林芝县| 红桥区| 白玉县| 天柱县| 枣庄市| 阳城县| 长乐市| 莒南县| 道孚县| 湘阴县| 盐津县| 陆良县| 农安县| 民乐县| 简阳市| 襄垣县| 彭州市| 肥乡县| 江山市| 古蔺县| 巴塘县| 公主岭市| 通海县| 赤水市| 丽江市|