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pEA974
pEA974
規(guī)格:
貨期:
編號:B208573
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 pEA974
商品貨號 B208573
Designations pEA974
Species Cricetus cricetus, hamster
Vector
Construct size (kb): 5.400000095367432
Insert
DNA: cDNA
Insert lengths(kb): 0.9739999771118164
Tissue: scrapie-infected brain
Gene product: prion protein (p27-30)(prion protein) [PRIP]
Alleles: A1, A1, A1, A1, A2, A2, B1, B1, B2, B2, A2, A2
Insert Size (kb) 0.974
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA distributed as 5 ug in 50 ul TE (volume: 50 ul,
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--4.4, 0.9; EcoRI--5.4; HindIII--5.4; BamHI--5.4; PstI--5.4.
Insert includes sequence from nt 2 to nt 808, with first in-frame ATG at nt 47, encoding a protein of 254 amino acids. Hybridization conditions suggest homology between hamster and human sequences is about 80%.
Hybridizes to a unique human genomic BamHI fragment of 10 kb (0.1X SSC, 68C) and human mRNA of about 3 kb.
Enzyme(s) not detecting polymorphism: ApaI, ApaLI, AvaI, AvuI, BanI, BamHI, BclI, BglI, BglII, BstEII, BstNI, EcoRI, EcoRV, HaeIII, HindIII, HinfI, KpnI, MspI, NheI, NruI, PstI, RsaI, SmaI, SstI, StuI, TaqI, XbaI, XmnI (20 unrelated European
References

Harris MS, et al. A BanI RFLP at a deletion hotspot in the human dystrophin gene. Nucleic Acids Res. 18: 385, 1990. PubMed: 1970164

Wu Y, et al. A PvuII RFLP detected in the human prion protein (PrP) gene. Nucleic Acids Res. 15: 3191, 1987. PubMed: 2882483

Robakis NK, et al. Localization of a human gene homologous to the PrP gene on the p arm of chromosome 20 and detection of PrP-related antigens in normal human brain. Biochem. Biophys. Res. Commun. 140: 758-765, 1986. PubMed: 2877664

Robakis NK, et al. Isolation of a cDNA clone encoding the leader peptide of prion protein and expression of the homologous gene in various tissues. Proc. Natl. Acad. Sci. USA 83: 6377-6381, 1986. PubMed: 3529083

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
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