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pc-fos (mouse)-3
pc-fos (mouse)-3
規(guī)格:
貨期:
編號(hào):B208519
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pc-fos (mouse)-3
商品貨號(hào) B208519
Designations pc-fos (mouse)-3
Species Mus musculus, mouse
Depositors T Curran
Applications
The 3' EcoRI/EcoRI fragment (5.4 kb) used in constructing this clone contains 3 of the 4 exons.
Vector
Construct size (kb): 11.19999980926514
Insert
DNA: genomic
Insert lengths(kb): 7.099999904632568
Tissue: liver/placenta
Gene product: FBJ murine osteosarcoma viral (v-fos) oncogene homolog( FBJ murine osteosarcoma viral (v-fos) oncogene homolog, FOS) [Fos]
Insert Size (kb) 7.100
Media ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried
Comments
Restriction digests analyzed on agarose gels give the following sizes (kb): Sst--5.1, 3.7, 2.8,0.4; XhoI--11.8; EcoRI--6.6, 5.4; EcoRI/Sst--4.4, 3.0, 2.0, 1.75, 0.56; EcoRI/SalI--6.0, 3.8, 1.8, 0.68.
The 3' EcoRI/EcoRI fragment (5.4 kb) used in constructing this clone contains 3 of the 4 exons.
Constructed from lambdac-fos(mouse)-2 (5' Sst/EcoRI) and pc-fos (mouse)-1 (3' EcoRI/EcoRI) to make a complete c-fos gene. The insert contains internal HindIII, XhoI, EcoRI, NcoI, SstI, SalI, and BamHI sites.
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Curran T, et al. Structure of the FBJ murine osteosarcoma virus genome: Molecular cloning of its associated helper virus and the cellular homolog of the v-fos gene from mouse and human cells. Mol. Cell. Biol. 3: 914-921, 1983. PubMed: 6306448

Miller AD, et al. c-fos protein can induce cellular transformation: a novel mechanism of activation of a cellular oncogene. Cell 36: 51-60, 1984. PubMed: 6607118

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