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熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > Microvascular Endothelial Cell Growth Kit-VEGF
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  • 0574-87157013
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  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
Microvascular Endothelial Cell Growth Kit-VEGF
Microvascular Endothelial Cell Growth Kit-VEGF
規(guī)格:
貨期:
編號:B208382
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板 


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      產品名稱
      Microvascular Endothelial Cell Growth Kit-VEGF
    
        
    
        
      商品貨號
      B208382
    
        
      
        
      Biosafety Level
      1 


        

        Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
        		
    
        
      
        
      Product Format
      frozen 1 kit
    
        
      
        
      Storage Conditions
      -20°C (or -70°C for long-term storage)
    
        
      
        
      Comments
      
        The Microvascular Endothelial Cell Growth Kit-VEGF contains components that when added to Vascular Cell Basal Medium (ATCC PCS-100-030) create a complete, low serum culture environment for microvascular endothelial cells isolated from normal human tissue. The final concentration for each component in complete endothelial growth medium is as follows:
        

          
    
        • rh VEGF: 5 ng/ml
          • 
    
        • rh EGF: 5 ng/ml
          • 
    
        • rh FGF basic: 5 ng/ml
          • 
    
        • rh IGF-1: 15 ng/ml
          • 
    
        • L-glutamine: 10 mM
          • 
    
        • Heparin sulfate: 0.75 Units/ml
          • 
    
        • Hydrocortisone: 1 µg/ml
          • 
    
        • Ascorbic acid: 50 µg/ml
          • 
    
        • Fetal bovine serum: 5% 
          • 

        

        		
    
        
      
        
      Complete Growth Medium
      
          
    
        1. Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
          2. 
    
        2. Thaw the components of the growth kit just prior to adding them to the basal medium. It is necessary to warm the L-glutamine component in a 37°C water bath and shake to dissolve any precipitates, prior to adding to the basal medium.
          3. 
    
        3. Obtain one bottle of Vascular Cell Basal Medium (475 mL) from cold storage.
          4. 
    
        4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
          5. 
    
        5. Using aseptic technique, and working in a laminar flow hood or biosafety cabinet transfer the volume of each growth kit component, as indicated in Table 1 or 2, to the bottle of basal medium using a separate sterile pipette for each transfer.
          6. 
    
          
    
          Table 1. If using the Microvascular Endothelial Cell Growth Kit-BBE (ATCC® PCS-110-040), add the indicated volume for each component:  
          
    
          
    
          
        
            
          
                 
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          Component
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
           
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          		
    
          
      
          
      Volume
      
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          Volume
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
           
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          		
    
          
      
          
      Sterility Tests
      
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          Final Concentration
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
           
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          		
    
          
      
          
      Functional Tests
      
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          Bovine Brain Extract (BBE)
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
           
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          		
    
          
      
          
      C of A
      
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          1.0 mL
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
           
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          
                
          		
    
          
              

          

						
					
					
          
							
          
							  
							  
							    
          
							      梅經理
							      17280875617
							      1438578920
							    
          
							    
          
							      胡經理
							      13345964880
							      2438244627
							    
          
							    
          
							      周經理
							      17757487661
							      1296385441
							    
          
							    
          
							      于經理
							      18067160830
							      2088210172
							    
          
							    
          
							      沈經理
							      19548299266
							      2662369050
							    
          
							    
          
							      李經理
							      13626845108
							      972239479
							    
          
							    
							            
							
          
					
          
				
				
          
					
				
          
			
			
		
		
          
	
  
          
	







巴中市|
蓬莱市|
四子王旗|
雷州市|
吴堡县|
宽甸|
开鲁县|
枣庄市|
施秉县|
陵川县|
平阳县|
柯坪县|
临夏市|
南投县|
象州县|
马公市|
克山县|
建宁县|
大庆市|
永定县|
确山县|
都昌县|
玛沁县|
阜宁县|
汾阳市|
呼伦贝尔市|
三明市|
永川市|
襄城县|
芒康县|
太康县|
鄂伦春自治旗|
汾阳市|
建宁县|
勃利县|
岱山县|
怀宁县|
肃宁县|
涿州市|
新沂市|
武汉市|


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