国产91亚洲福利精品一区二区,国产综合成人久久大片91,国产成人精品久久综合,久久久91精品国产一区二区三区,91福利国产在线在线播放,91精品国产高清久久久久久91,91精品国产福利在线观看麻豆,国产免费一区二区三区在线观看

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > Microvascular Endothelial Cell Growth Kit-VEGF
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
Microvascular Endothelial Cell Growth Kit-VEGF
Microvascular Endothelial Cell Growth Kit-VEGF
規(guī)格:
貨期:
編號(hào):B208382
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板 


<p id="5djiv"></p>

  
    

      產(chǎn)品名稱
      Microvascular Endothelial Cell Growth Kit-VEGF
    

    

      商品貨號(hào)
      B208382
    

      

      Biosafety Level
      1 




Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

    

      

      Product Format
      frozen 1 kit
    

      

      Storage Conditions
      -20°C (or -70°C for long-term storage)
    

      

      Comments
      
The Microvascular Endothelial Cell Growth Kit-VEGF contains components that when added to Vascular Cell Basal Medium (ATCC PCS-100-030) create a complete, low serum culture environment for microvascular endothelial cells isolated from normal human tissue. The final concentration for each component in complete endothelial growth medium is as follows:


    
    
  • rh VEGF: 5 ng/ml
    • 
    
  • rh EGF: 5 ng/ml
    • 
    
  • rh FGF basic: 5 ng/ml
    • 
    
  • rh IGF-1: 15 ng/ml
    • 
    
  • L-glutamine: 10 mM
    • 
    
  • Heparin sulfate: 0.75 Units/ml
    • 
    
  • Hydrocortisone: 1 µg/ml
    • 
    
  • Ascorbic acid: 50 µg/ml
    • 
    
  • Fetal bovine serum: 5% 
    • 




    

      

      Complete Growth Medium
      
    
    
  1. Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
    2. 
    
  2. Thaw the components of the growth kit just prior to adding them to the basal medium. It is necessary to warm the L-glutamine component in a 37°C water bath and shake to dissolve any precipitates, prior to adding to the basal medium.
    3. 
    
  3. Obtain one bottle of Vascular Cell Basal Medium (475 mL) from cold storage.
    4. 
    
  4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
    5. 
    
  5. Using aseptic technique, and working in a laminar flow hood or biosafety cabinet transfer the volume of each growth kit component, as indicated in Table 1 or 2, to the bottle of basal medium using a separate sterile pipette for each transfer.
    6. 
    
    
    
    Table 1. If using the Microvascular Endothelial Cell Growth Kit-BBE (ATCC® PCS-110-040), add the indicated volume for each component:  
    
    
    
    
    
        
            
    
                 
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    Component
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
     
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    		
    
    
      
    
      Volume
      
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    Volume
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
     
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    		
    
    
      
    
      Sterility Tests
      
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    Final Concentration
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
     
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    		
    
    
      
    
      Functional Tests
      
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    Bovine Brain Extract (BBE)
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
     
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    		
    
    
      
    
      C of A
      
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    1.0 mL
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
     
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    
                
    		
    
    
        

    

						
					
					
    
							
    
							  
							  
							    
    
							      梅經(jīng)理
							      17280875617
							      1438578920
							    
    
							    
    
							      胡經(jīng)理
							      13345964880
							      2438244627
							    
    
							    
    
							      周經(jīng)理
							      17757487661
							      1296385441
							    
    
							    
    
							      于經(jīng)理
							      18067160830
							      2088210172
							    
    
							    
    
							      沈經(jīng)理
							      19548299266
							      2662369050
							    
    
							    
    
							      李經(jīng)理
							      13626845108
							      972239479
							    
    
							    
							      
							
    
					
    
				
				
    
					
				
    
			
			
		
		
    
	
  
    
	







南江县|
太原市|
本溪市|
邹城市|
伊春市|
定兴县|
永仁县|
故城县|
兰溪市|
屯门区|
乐山市|
孟津县|
盐边县|
沧源|
宁陕县|
名山县|
巨鹿县|
稻城县|
墨竹工卡县|
芜湖市|
军事|
班玛县|
岢岚县|
道真|
承德县|
峨边|
额敏县|
贡觉县|
青冈县|
许昌市|
辉南县|
乐清市|
卢氏县|
义马市|
阜新市|
吴忠市|
通渭县|
昌都县|
深州市|
深州市|
长乐市|


<bdo id="5djiv"></bdo>
    <bdo id="5djiv"></bdo>