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熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > Microvascular Endothelial Cell Growth Kit-VEGF
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  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
Microvascular Endothelial Cell Growth Kit-VEGF
Microvascular Endothelial Cell Growth Kit-VEGF
規(guī)格:
貨期:
編號:B208382
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板 


      • 
  
    
      
      產(chǎn)品名稱
      Microvascular Endothelial Cell Growth Kit-VEGF
    
      
    
      
      商品貨號
      B208382
    
      
      
      
      Biosafety Level
      1 


      

      Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
      		
    
      
      
      
      Product Format
      frozen 1 kit
    
      
      
      
      Storage Conditions
      -20°C (or -70°C for long-term storage)
    
      
      
      
      Comments
      
      The Microvascular Endothelial Cell Growth Kit-VEGF contains components that when added to Vascular Cell Basal Medium (ATCC PCS-100-030) create a complete, low serum culture environment for microvascular endothelial cells isolated from normal human tissue. The final concentration for each component in complete endothelial growth medium is as follows:
      

        
    
      • rh VEGF: 5 ng/ml
        • 
    
      • rh EGF: 5 ng/ml
        • 
    
      • rh FGF basic: 5 ng/ml
        • 
    
      • rh IGF-1: 15 ng/ml
        • 
    
      • L-glutamine: 10 mM
        • 
    
      • Heparin sulfate: 0.75 Units/ml
        • 
    
      • Hydrocortisone: 1 µg/ml
        • 
    
      • Ascorbic acid: 50 µg/ml
        • 
    
      • Fetal bovine serum: 5% 
        • 

      

      		
    
      
      
      
      Complete Growth Medium
      
        
    
      1. Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
        2. 
    
      2. Thaw the components of the growth kit just prior to adding them to the basal medium. It is necessary to warm the L-glutamine component in a 37°C water bath and shake to dissolve any precipitates, prior to adding to the basal medium.
        3. 
    
      3. Obtain one bottle of Vascular Cell Basal Medium (475 mL) from cold storage.
        4. 
    
      4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
        5. 
    
      5. Using aseptic technique, and working in a laminar flow hood or biosafety cabinet transfer the volume of each growth kit component, as indicated in Table 1 or 2, to the bottle of basal medium using a separate sterile pipette for each transfer.
        6. 
    
        
    
        Table 1. If using the Microvascular Endothelial Cell Growth Kit-BBE (ATCC® PCS-110-040), add the indicated volume for each component:  
        
    
        
    
        
        
            
        
                 
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
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      Volume
      
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
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
      Sterility Tests
      
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        Final Concentration
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
         
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        		
    
        
      
        
      Functional Tests
      
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        Bovine Brain Extract (BBE)
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
         
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        		
    
        
      
        
      C of A
      
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        1.0 mL
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
         
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        		
    
        
            

        

						
					
					
        
							
        
							  
							  
							    
        
							      梅經(jīng)理
							      17280875617
							      1438578920
							    
        
							    
        
							      胡經(jīng)理
							      13345964880
							      2438244627
							    
        
							    
        
							      周經(jīng)理
							      17757487661
							      1296385441
							    
        
							    
        
							      于經(jīng)理
							      18067160830
							      2088210172
							    
        
							    
        
							      沈經(jīng)理
							      19548299266
							      2662369050
							    
        
							    
        
							      李經(jīng)理
							      13626845108
							      972239479
							    
        
							    
							          
							
        
					
        
				
				
        
					
				
        
			
			
		
		
        
	
  
        
	







翼城县|
中卫市|
西峡县|
哈巴河县|
葫芦岛市|
罗江县|
定安县|
乌拉特中旗|
剑川县|
怀柔区|
百色市|
泰来县|
越西县|
石景山区|
鄂尔多斯市|
琼中|
文成县|
三穗县|
渑池县|
文登市|
乌恰县|
邵东县|
琼海市|
钟山县|
大石桥市|
天祝|
定陶县|
碌曲县|
山东|
琼结县|
合阳县|
综艺|
灵台县|
枣强县|
双桥区|
理塘县|
左权县|
合川市|
密山市|
永城市|
资中县|