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pUC18CMR
pUC18CMR
規(guī)格:
貨期:
編號(hào):B208227
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pUC18CMR
商品貨號(hào) B208227
Designations pUC18CMR
Depositors HP Schweizer
Biosafety Level 1
Vector Information
Size (kb): 4.2729997634887700
Vector: pUC18CMR (plasmid)
Promoters: Promoter none
Construction: pUC18, pUC4K, pBR325
Marker(s):ampR,cmlR
Construct size (kb): 4.27299976348877
Features: marker(s): ampR, cmlR
promoter: none
replicon: pMB1
terminator: none
enhancer: none
Applications
contains easily purifiable cassette(s) for construction
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.4, 0.89; PstI--4.27; EcoRI/HindIII--2.45, 0.89, 0.75.
The cml cassette can be isolated by digestion with one enzyme (BamHI), by double digestion yielding blunt ends (HincII/SmaI), or by double digestion yielding overhangs for directional cloning.
pUC18CML (ATCC 37717) and pUC18CMR (ATCC 37718) differ only in the orientation of the cml cassette. In pUC18CMR, the lac promoter and the cml gene are in the same orientation.
A plasmid containing the gene determining chloramphenicol resistance which is flanked by polylinker sites for ease in manipulation.
cmlR sequences (pBR325 cut with BclI/blunt ended with Klenow/cut with HincII) were ligated to a 2.6 kb HincII fragment of pUC4K. The 1.6 kb BamHI fragment was cloned into M13mp18; a 1.6 kb KpnI/HindIII fragment was cloned into the BamHI site of pUC18.
Media ATCC® Medium 1235: LB Medium (ATCC medium 1065) with 5 mcg/ml tetracycline and 10 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Schweizer HP. The pUC18CM plasmids: a chloramphenicol resistance gene cassette for site-directed insertion and deletion mutagenesis in Escherichia coli. BioTechniques 8: 612-616, 1990. PubMed: 2192739

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