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pCLL2201
pCLL2201
規(guī)格:
貨期:
編號(hào):B208156
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) pCLL2201
商品貨號(hào) B208156
Designations pCLL2201
GenBank Number

M63556

Species Bacteroides fragilis (Veillon and Zuber) Castellani and Chalmers
Depositors American Cyanamid Co., BA Rasmussen, American Cyanamid Co.
Applications
in another host, produces protein metallo-beta-lactamase
Vector
Construct size (kb): 10.10000038146973
Insert
DNA: genomic
Insert lengths(kb): 6.5
Gene product: metallo-beta-lactamase [ccrA]
Target Gene: metallo-beta-lactamase
Insert Size (kb) 6.5
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.6, 3.5; HindIII--6.0, 1.9, 1.4, 0.8; PstI--6.3, 3.8; KpnI--10.1.
The insert contains the following restriction sites (approximate kb from the end upstream of ccrA): PstI--0.5, MboI--0.9, 1.9; KpnI--1.2; HindIII--3.6, 5.4.
The 941 bp MboI fragment contains the complete gene, with 150 bp 5' and 50 bp 3' flanking sequence. The sequence of most of this fragment has been reported.
The vector is a derivative of pMK16 deleted for the carboxy terminal half of the tetR marker and has had the small EcoRI/BamHI fragment replaced with the MCS-containing EcoRI/BamHI fragment from plink 322.
References

Rasmussen BA, et al. Escherichia coli chromosomal mutations that permit direct cloning of the Bacteroides fragilis metallo-beta-lactamase gene, ccrA. Mol. Microbiol. 5: 1211-1219, 1991. PubMed: 1956298

Rasmussen BA, et al. Cloning and sequencing of the class B beta-lactamase gene (ccrA) from Bacteroides fragilis TAL3636. Antimicrob. Agents Chemother. 34: 1590-1592, 1990. PubMed: 2121094

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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