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pVC33
pVC33
規(guī)格:
貨期:
編號:B208152
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pVC33
商品貨號 B208152
Designations pVC33
Species Homo sapiens, human
Depositors NIH, I Pastan, NIH
Applications
produces protein exotoxin A
produces protein transforming growth factor, alpha transforming growth factor alpha
Vector
Construct size (kb): 4.0
Insert
DNA: cDNA
Insert lengths(kb): 1.379999995231628
Gene product: transforming growth factor, alpha(transforming growth factor alpha) [TGFA]
Alleles: A1, A2, B1, B2
Target Gene: exotoxin A, transforming growth factor, alpha
Insert Size (kb) 1.380
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
The construct contains unique StuI, EcoRI, and NdeI sites.
Restriction digests of the clone give the following sizes (kb): EcoRI--4.0; BamHI/BglII--3.2, 0.88; NdeI--4.0.
When expressed in Escherichia coli B121, this plasmid produces a protein that reacts with antibodies to transforming growth factor alpha and to Pseudomonas exotoxin A. This fusion protein has a molecular weight of 51 kDa.
This was constructed by deleting some non-coding sequences from JH8 (ATCC 67208), adding an oligonucleotide containing a Tth111I site, and inserting a 152 bp Fnu4HI fragment from phTGF10-925 encoding TNFA into the Tth111I site.
References

Pastan IH, et al. Recombinant Pseudomonas exotoxins: construction of an active immunotoxin with low side effects. US Patent 4,892,827 dated Jan 9 1990

Ira Pastan, personal communication

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
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