| 產(chǎn)品名稱(chēng) |
pVC33 |
| 商品貨號(hào) |
B208152 |
| Designations |
pVC33 |
| Species |
Homo sapiens, human |
| Depositors |
NIH, I Pastan, NIH |
| Applications |
produces protein exotoxin A produces protein transforming growth factor, alpha transforming growth factor alpha |
| Vector |
Construct size (kb): 4.0 |
| Insert |
DNA: cDNA Insert lengths(kb): 1.379999995231628 Gene product: transforming growth factor, alpha(transforming growth factor alpha) [TGFA] Alleles: A1, A2, B1, B2 Target Gene: exotoxin A, transforming growth factor, alpha |
| Insert Size (kb) |
1.380 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
The construct contains unique StuI, EcoRI, and NdeI sites. Restriction digests of the clone give the following sizes (kb): EcoRI--4.0; BamHI/BglII--3.2, 0.88; NdeI--4.0. When expressed in Escherichia coli B121, this plasmid produces a protein that reacts with antibodies to transforming growth factor alpha and to Pseudomonas exotoxin A. This fusion protein has a molecular weight of 51 kDa. This was constructed by deleting some non-coding sequences from JH8 (ATCC 67208), adding an oligonucleotide containing a Tth111I site, and inserting a 152 bp Fnu4HI fragment from phTGF10-925 encoding TNFA into the Tth111I site. |
| References |
Pastan IH, et al. Recombinant Pseudomonas exotoxins: construction of an active immunotoxin with low side effects. US Patent 4,892,827 dated Jan 9 1990
Ira Pastan, personal communication
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
