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pG59/T1 [C151/T1, C151/L1-T1]
pG59/T1 [C151/T1, C151/L1-T1]
規(guī)格:
貨期:
編號:B208107
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pG59/T1 [C151/T1, C151/L1-T1]
商品貨號 B208107
Designations pG59/T1 [C151/T1, C151/L1-T1]
GenBank Number

J03496

Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors A Tzagoloff
Vector
Construct size (kb): 21.70000076293945
Insert
DNA: genomic
Insert lengths(kb): 11.0
Gene product: leucyl tRNA synthetase, mitochondrial [MSL1]
Insert Size (kb) 11.0
Genotype MATalpha leu2-3 leu2-112 msl1-1
Mating Type alpha
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
The insert contains the following sites separated by (approximate kb): BamHI- 0.24- PstI- 0.45- BamHI- 1.0- BamHI- 0.63- XhoI- 0.29- PstI- 2.3- XbaI- 1.0- BamHI- 0.6- XbaI- 1.1- XbaI- 2.9- BamHI- 0.25- BamHI.
Restriction digests of the clone give the following sizes (kb): EcoRI/SalI--11, 3.7, 3.3, 2.2, 1.05, 0.94; KpnI--uncut; XhoI--15, 7; XbaI--16, 4.2, 1.45; PvuII--17, 5.1.
The insert includes a continuous open reading frame of 2682 nt encoding a protein of 894 amino acids and a molecular weight of 102 kDa.
The probe, pG59/T1, has been deposited in Escherichia coli RR1 as ATCC 37668 and in Saccharomyces cerevisiae C151/L1 as ATCC 66312.
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References

Tzagoloff A, et al. Homology of yeast mitochondrial leucyl-tRNA synthetase and isoleucyl- and methionyl-tRNA synthetases of Escherichia coli. J. Biol. Chem. 263: 850-856, 1988. PubMed: 2826465

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