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pAX-7
pAX-7
規(guī)格:
貨期:
編號(hào):B243393
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pAX-7
商品貨號(hào) B243393
Designations pAX-7
Applications
Enzyme(s) not detecting polymorphism: BamHI, BglII, EcoRI, EcoRV, HindIII, MspI, PstI, TaqI. Tested against the X chromosome of 40 Sardinian males.
Vector
Construct size (kb): 4.75
Insert
DNA: cDNA
Insert lengths(kb): 1.049999952316284
Tissue: HeLa cell line
Gene product: DNA Segment, numerous copies [DNF3]
Alleles: A2, A1
Insert Size (kb) 1.050
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA distributed as 5 ug in 50 ul TE (volume: 50 ul,
Comments
Restriction digests of the clone give the following sizes (kb): PstI--3.7, 1.05, 0.25, 0.1; BamHI--4.7; EcoRI--4.7; AvaI--2.9, 1.7, 0.2.
Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with.
The SstI polymorphism has been mapped to the X chromosome and has been studied in 15 families.
The insert contains an internal PstI site which results in two fragments, 1000 bp pAX-7-1 and 300 bp pAX-7-2. For EcoRI digested human DNA, pAX-7-1 hybridizes with 4 fragments and pAX-7-2 hybridizes with only 2 fragments.
Enzyme(s) not detecting polymorphism: BamHI, BglII, EcoRI, EcoRV, HindIII, MspI, PstI, SacI, TaqI. For chromosomes 12, 18, and 21 of 15 Sardinian males.
Enzyme(s) not detecting polymorphism: BamHI, BglII, EcoRI, EcoRV, HindIII, MspI, PstI, TaqI. Tested against the X chromosome of 40 Sardinian males.
References

Balazs I, et al. Isolation and characterization of human random cDNA clones homologous to DNA from the X chromosome. Somatic Cell Mol. Genet. 10: 385-397, 1984. PubMed: 6087473

Barbara Weiffenbach, personal communication

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于經(jīng)理 18067160830 2088210172
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