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Primary Epidermal Keratinocytes; Normal, Human, Neonatal Foreskin (HEKn)
Primary Epidermal Keratinocytes; Normal, Human, Neonatal Foreskin (HEKn)
規(guī)格:
貨期:
編號(hào):B238796
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


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    產(chǎn)品名稱 Primary Epidermal Keratinocytes; Normal, Human, Neonatal Foreskin (HEKn)
    商品貨號(hào) B238796
    Organism Homo sapiens, human
    Tissue Skin
    Cell Type Keratinocyte
    Morphology Cobblestone appearance; cells are rounded, not flat; cells display a high mitotic index; at near 80% confluence, the cells will be associated with each other in colonies.
    Growth Properties Adherent
    Biosafety Level 1

    [These primary cells are not known to harbor an agent recognized to cause disease in healthy adult humans. Handle as a potentially biohazardous material under at least Biosafety Level 1 containment. Cells derived from primate lymphoid tissue may fall under the regulations of 29 CFR 1910.1030 Bloodborne Pathogens.  

    ATCC recommends that appropriate safety procedures be used when handling all primary cells and cell lines, especially those derived from human or other primate material. Detailed discussions of laboratory safety procedures are provided in Laboratory Safety: Principles and Practice, 2nd ed. (ASM Press, Washington, DC) (Fleming et al., 1995) and Caputo, J.L. Biosafety procedures in cell culture. (1988) J. Tissue Culture Methods 11:223.

    Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS Publication No. (CDC) 93-8395. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.]

    Human Material Precaution 

    All tissues used for isolation are obtained under informed consent and conform to HIPAA standards to protect the privacy of the donor’s personal health information. It is best to use caution when handling any human cells. We recommend that all human cells be accorded the same level of biosafety consideration as cells known to carry HIV. With infectious virus assays or viral antigen assays, even a negative test result may leave open the possible existence of a latent viral genome.


    Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

    Disease Normal
    Age Neonatal
    Gender Male
    Applications
    Toxicology, wound repair, skin cancer, response to UV radiation, psoriasis, eczema, viral infection, gene delivery systems, cellular differentiation
    Product Format frozen 1 mL
    Storage Conditions -130°C or below
    Images Primary Keratinocytes 11 Day Differentiation
    Comments
    A complete solution to propagate neonatal keratinocytes. The serum-free media is formulated to inhibit fibroblast growth, and the low calcium concentration (60 µM) slows differentiation. No feeder layers, extracellular matrix proteins or other substrates are required.
    Complete Growth Medium
    1. Obtain one Keratinocyte Growth Kit from the freezer; make sure that the caps of all components are tight.
    2. Thaw the components of the growth kit just prior to adding them to the basal medium. It is necessary to warm the L-glutamine component in a 37°C water bath and shake to dissolve any precipitates prior to adding to the basal medium.
    3. Obtain one bottle of Dermal Cell Basal Medium (485 mL) from cold storage.
    4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol. 
    5. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the indicated volume of each growth kit component, as indicated in Table 1, to the bottle of basal medium using a separate sterile pipette for each transfer.
    6.        Table 1. Keratinocyte Growth Kit Components 

      Component

      Subculturing

      Volume

      Volume

      Final Concentration

      Cells per Vial

      Bovine Pituitary Extract (BPE)

      Sterility Tests

      2.0 mL

      Viral Testing

      0.4%

      Viability

      rh TGF-a

      C of A

      0.5 mL

      梅經(jīng)理 17280875617 1438578920
      胡經(jīng)理 13345964880 2438244627
      周經(jīng)理 17757487661 1296385441
      于經(jīng)理 18067160830 2088210172
      沈經(jīng)理 19548299266 2662369050
      李經(jīng)理 13626845108 972239479
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