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GC-2.2 [GCS-2kb, lambda-EZZ-gamma3]
GC-2.2 [GCS-2kb, lambda-EZZ-gamma3]
規(guī)格:
貨期:
編號:B231735
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 GC-2.2 [GCS-2kb, lambda-EZZ-gamma3]
商品貨號 B231735
Designations GC-2.2 [GCS-2kb, lambda-EZZ-gamma3]
GenBank Number

M11080

Species Homo sapiens, human
Applications
in another host, produces protein glucosidase, beta; acid Glucosidase, beta, acid, glucocerebrosidase
Vector
Construct size (kb): 6.599999904632568
Insert
DNA: cDNA
Insert lengths(kb): 2.200000047683716
Tissue: fibroblast WI-38 cell line
Gene product: glucosidase, beta; acid(Glucosidase, beta, acid, glucocerebrosidase) [GBA]
Target Gene: glucosidase, beta; acid
Insert Size (kb) 2.200
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.0, 1.7, 1.0; EcoRI--4.4, 2.3; HindIII--4.6, 2.1; PvuII--3.7, 3.1; SalI--5.2, 1.6.
The insert includes the following restriction sites (kb from the 5' end): BamHI--0.027, 1.629; HindIII--0.231; ScaI--1.022; SalI--1.403.
Contains the complete coding sequence but lacks some of the 5' untranslated region. The coding sequences extend from nt 154 to 1709 of the published sequence.
Constructed from G5A-IY and ID9bb by taking advantage of the unique ScaI site in the region of overlap.
References

Zimran A, et al. Linkage of the PvuII polymorphism with the common Jewish mutation for gaucher disease. Am. J. Hum. Genet. 46: 902-905, 1990. PubMed: 1971142

Dahl N, et al. Gaucher disease type III (Norrbottnian type) is caused by a single mutation in exon 10 of the glucocerebrosidase gene. Am. J. Hum. Genet. 47: 275-278, 1990. PubMed: 2378352

Sorge J, et al. Molecular cloning and nucleotide sequence of human glucocerebrosidase cDNA. Proc. Natl. Acad. Sci. USA 82: 7289-7293, 1985. PubMed: 3864160

Sorge J, et al. Correction. Proc. Natl. Acad. Sci. USA 83: 3567, 1986.

Sorge JA, et al. The human glucocerebrosidase gene has two funtional ATC initiator codons. Am. J. Hum. Genet. 41: 1016-1024, 1987. PubMed: 3687939

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