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Size (kb): 5.9000000953674320

Vector: pSH (plasmid)

Promoters: Promoter T3

Construction: pEN1, BLpSV

Marker(s):ampR

Construct size (kb): 5.900000095367432

Features: marker(s): ampR
promoter: HTLV-1 LTR, T7, T3
replicon: pMB1, SV40
terminator: SV40 polyadenylation
enhancer: HIV-1 LTR

expression vector

shuttle vector

Restriction digests of the clone give the following sizes (kb): EcoRI--5.9; HindIII--5.9; PstI--4.3, 1.6.

Shuttle expression vector utilizing a chimeric promoter derived from the HTLV-I LTR and the HIV-1 LTR. Supports high levels of expression in human and mouse cells.

The order of the major features in the plasmid is: T7 promoter - XhoI - SV40 promoter - HTLV-1 LTR promoter - HIV-1 LTR enhancer - EcoRI/MCS/BglII - SV40 polyadenylation - T3 promoter - pMB1 ori - ampR.

Temperature: 30.0°C