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produces protein proprotein convertase subtilisin/kexin type 1 neuroendocrine convertase 1 (pro-protein-convertase PC1)

Construct size (kb): 6.099999904632568

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-3Zf(+)
Intact vector size: 3.199
Type of vector: phagemid
Vector end: XbaI
Vector end: XbaI
Cloning sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI
SphI HindIII
Polylinker sites: T7-EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII
PstI SphI HindIII-SP6
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
promoter: lac, SP6, T7
replicon: pMB1, f1
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PCSK1
Other gene symbols: NEC1
Genomic copy number: unique
Gene name: proprotein convertase subtilisin/kexin type 1
Contains complete coding sequence?: Y
Chromosome: 5; Localization: 5 q15-q21
Tissue: lung
Type of DNA: cDNA
Insert end: XbaI
Insert end: XbaI
Insert size (kb): 2.9
Cross references: DNA Seq. Acc.: X64810

Insert lengths(kb): 2.900000095367432

Tissue: lung

Gene product: proprotein convertase subtilisin/kexin type 1(neuroendocrine convertase 1 (pro-protein-convertase PC1)) [NEC1]

Target Gene: proprotein convertase subtilisin/kexin type 1

Restriction digests of the clone give the following sizes (kb): HindIII/XbaI--3.2, 2.9; BamHI--4.1, 2.0; EcoRI--3.5, 1.9, 0.65; PstI--3.3, 2.8; SacI--3.6, 2.6.

The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.12; EcoRI--1.82; BamHI--1.97.

The 3' XbaI site is not cleaved by XbaI. Sequence analysis indicates that the site is present.

Corresponds to nt 87-3039 of the sequence record. Contains the complete coding sequence (nt 190-2451 of the sequence record).