Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--31.0, 12.5; HindIII--22.0, 8.4, 4.4, 3.5, 2.9, 2.3, 1.3; SalI--20.5, 13.5, 9.0. The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--10.0; EcoRI--6.4; PstI--8.2, 8.7, 12.4; HindIII--0.8, 3.6, 7.1, 8.4, 10.6. |