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pCN61
pCN61
規(guī)格:
貨期:
編號:B220905
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 pCN61
商品貨號 B220905
Designations pCN61
Depositors R Diaz Orejas
Biosafety Level 1
Vector Information
Size (kb): 8.1000003814697270
Vector: pCN61 (plasmid)
Construction: pCN60
Marker(s):ampR,strR
Construct size (kb): 8.100000381469727
Features: marker(s): ampR, strR
replicon: repA, pMB1
Applications
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/KpnI--4.1, 3.7; HindIII--8.2; SmaI--8.2; BamHI/SmaI--6.7, 1.2.
Copy number is 2-3 per genome in Pseudomonas spp. Stability is reduced compared to pCN40 (ATCC 77097).
Contains the basic replicon of pPS10 which has been sequenced (EMBL accession number X58896). The repA portion is flanked by EcoRI sites (nt 536 and 1500), with the site at nt 536 between the repA promoter and coding sequences.
Retains the mobilization site of pBR322-derived vectors and can be mobilized if suitable functions are provided in trans.
Shuttle vector permitting maintaining recombinants at low copy number and, if necessary, curing the host of recombinants.
Constructed by deleting a 0.6 kb HindIII fragment from pCN60 (ATCC 77101) which included the multiple cloning site and 0.2 kb (nt 1615-1823) from the pPS10 replicon.
The order of the major features in this plasmid is: KpnI - repA - strR - PstI - SmaI - BamHI - HindIII - bla - pMB1 ori.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Nieto C, et al. Cloning vectors, derived from a naturally occurring plasmid of Pseudomonas savastanoi, specifically tailored for genetic manipulations in Pseudomonas. Gene 87: 145-149, 1990. PubMed: 2110095

Ramon Diaz Orejas, personal communication

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周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
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