| 產(chǎn)品名稱 |
pCMV5.4-6 |
| 商品貨號(hào) |
B219717 |
| Designations |
pCMV5.4-6 |
| GenBank Number |
J00136
|
| Species |
Homo sapiens, human |
| Depositors |
W Cheung |
| Applications |
in another host, produces protein coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) |
| Vector |
Construct size (kb): 7.5 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pCMV5
Intact vector size: 4.700
Type of vector: phagemid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI BamHI HindIII
Polylinker sites:
Host range: mammalian cells; Escherichia coli
Features (with orientation and position when available):
promoter: CMV, ->
terminator: hGH, ->
replicon: SV40
replicon: pMB1
marker(s): ampR, <-
replicon: f1, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: F9
Genomic copy number: unique
Gene name: coagulation factor IX (plasma thromboplastic component,
Christmas disease, hemophilia B)
Contains complete coding sequence?: U
Chromosome: X; Localization: X q26.3-q27.1
Type of DNA: cDNA
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 2.8
Cross references: DNA Seq. Acc.: J00136 Insert lengths(kb): 2.799999952316284 Gene product: coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) [F9] Alleles: A1, A2, D2, C1, D1, C2 Target Gene: coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) |
| Insert Size (kb) |
2.800 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--4.9, 2.8; BglII--7.6; EcoRI--4.9, 2.6; NotI--7.6; SstI--7.0, 0.56. Digestion with EcoRI produces fragments of 5.0 and 2.5 kb. Bacteria may grow slowly due to the high plasmid copy number. Encodes the following mutant form: G4F, K5-. The glycine and lysine on residues 4 and 5 of human factor IX were replaced with one phenylalanine. |
| References |
Andersson S, et al. Cloning, structure, and expression of a mitochondrial cytochrome P-450 sterol 26-hyroxylase, a bile acid biosnythetic enzyme. J. Biol. Chem. 264: 8222-8229, 1989. PubMed: 2722778
Cheung WF, et al. The binding of human factor IX to endothelial cells is mediated by residues 3-11. J. Biol. Chem. 267: 20529-20531, 1992. PubMed: 1400370
Cheung WF, et al. The role of the epidermal growth factor-1 and hydrophobic stack domains of human factor IX in binding to endothelial cells. J. Biol. Chem. 266: 8797-8800, 1991. PubMed: 1851159
Wing-Fai Cheung, personal communication
|
