This material is available under the condition that it will not be used for commercial purpose or distributed to third parties.
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen
Type Strain
no
Genotype
MATa ura3-52 leu2delta1 his3delta200 trp1delta63 snf3::hisG rgt2::HIS3
RefGanster RW, et al. Identification of a calcineurin-independent pathway required for sodium ion stress response in Saccharomyces cerevisiae. Genetics 150: 31-42, 1998. PubMed: 9725828
Preceptrol®
no
Mating Type
a
Ploidy
haploid
Comments
This material is available under the condition that it will not be used for commercial purpose or distributed to third parties.
MSY402 grows poorly on glucose as sole carbon source. Culture the strain on glycerol-ethanol media to avoid spontaneous mutants with enhanced growth.
MSY402 and MSY404 must be grown under selection for Ura or the URA3 gene is lost by recombination between the flanking hisG cassettes.
Medium
ATCC® Medium 1904: Synthetic complete medium
Growth Conditions
Temperature: 25.0°C
Protocol: with glycerol, without glucose
Subcultivation
Protocol: with glycerol, without glucose
Name of Depositor
M Schmidt
Special Collection
NCRR Contract
Isolation
MSY402 grows poorly on glucose as sole carbon source. Culture the strain on glycerol-ethanol media to avoid spontaneous mutants with enhanced growth.
References
Ganster RW, et al. Identification of a calcineurin-independent pathway required for sodium ion stress response in Saccharomyces cerevisiae. Genetics 150: 31-42, 1998. PubMed: 9725828
Martin Schmidt, personal communication
Ganster RW, et al. Identification of a calcineurin-independent pathway required for sodium ion stress response in Saccharomyces cerevisiae. Genetics 150: 31-42, 1998. PubMed: 9725828