ATCC® Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load.
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen Specification range: 1 x 105 to 1 x 106 copies/µL 100 μL per vial with Biomatrica RNAstable
Storage Conditions
-70°C or colder
Intended Use
The synthetically engineered sequence of the product constitutes intellectual property belonging to ATCC. Unauthorized use, including sequencing, modification, or reverse-engineering, of the product is expressly prohibited without prior ATCC consent.
Comments
Manufactured under ISO 13485 guidance
Preparation includes fragments from the RNA-dependent RNA polymerase, VP1 (ORF1-ORF2 junction), and VP2 regions.
The following primers and probe can be used with this nucleic acid preparation RefVega E, et al. Novel surveillance network for norovirus gastroenteritis outbreaks, United States. Emerg. Infect. Dis. 17(8): 1389-1395, 2011. PubMed: 21801614:
Forward primer: CARGARBCNATGTTYAGRTGGATGAG
Reverse primer: TCGACGCCATCTTCATTCACA
Probe: FAM-TGGGAGGGCGATCGCAATCT-BHQ
Product Availability: ATCC® VR-3235SD is an updated version of ATCC® VR-3200SD that includes quantitated genome copies/µL and has been modified to account for customer feedback regarding various assay compatibilities. ATCC® VR-3200SD will be available while supplies last.
Special Collection
RNA
References
Vega E, et al. Novel surveillance network for norovirus gastroenteritis outbreaks, United States. Emerg. Infect. Dis. 17(8): 1389-1395, 2011. PubMed: 21801614
Le Guyader FS, et al. Detection and quantification of noroviruses in shellfish. Appl. Environ. Microbiol. 75(3): 618-624, 2009. PubMed: 19047383