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pSELECT-1 [pALTER-1]
pSELECT-1 [pALTER-1]
規(guī)格:
貨期:
編號:B213302
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pSELECT-1 [pALTER-1]
商品貨號 B213302
Designations pSELECT-1 [pALTER-1]
Depositors Promega Corporation, JC Grosch, Promega Corporation
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level 1
Vector Information
Size (kb): 5.6799998283386230
Vector: pSELECT-1 (phagemid)
Promoters: Promoter T7
Construction: pBR322, pGEM-3Zf(+)
Marker(s):tetR
Construct size (kb): 5.679999828338623
Features: insert detection: lacZ'
marker(s): tetR
promoter: lac, SP6, T7
replicon: pMB1, f1
Applications
vector containing primer sites useful for sequencing
vector permitting RNA synthesis in vitro
vector permitting visual detection of recombinants
vector useful for in-vitro mutagenesis
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.2; BamHI--6.2; HindIII--6.2.
This vector contains the following unique restriction sites: ClaI, EcoRV, StyI, ScaI and AatII.
Useful for in vitro mutagenesis experiments. Encodes the beta-galactosidase alpha peptide, permitting blue-white visual detection. Can be used to produce ssDNA. Contains promoters for in vitro RNA synthesis and priming sites useful for sequencing.
Contains an inactive ampicillin resistance gene which can be reverted to functional expression by a repair oligonucleotide.
Media ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions
Temperature: 37.0°C
References

Promega Corporation, personal communication

Shipped frozen
Shipping Information

Shipped: ?Freeze dried E. coli containing the plasmid

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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