ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
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Purified plasmid DNA: 45050D
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
Full-length (353 bp) Chrysanthemum Stunt Viroid (CSV) cDNA with BamHI ends was cloned into BamHI of pUC9. Constructed by T. Candresse.
Noninfectious even when cDNA insert is released with BamHI. Contains unknown lethal alteration(s).
The insert contains internal HindIII and PstI sites.
Restriction digests of the clone give the following sizes (kb): BamHI--2.65, 0.35; PstI--3.1; EcoRI--3.1.